不同剂量阿托伐他汀对稳定性心绞痛患者TNF-α 和LDL-C 水平的影响

目的:观察不同剂量阿托伐他汀对冠心病稳定性心绞痛患者血清肿瘤坏死因子a(TNF-α)和低密度脂蛋白胆固醇(LDL-C)水平的影响。方法:用酶联免疫吸附法和生化发光分析法测定40例正常对照组和40例阿托伐他汀10mg组及39例阿托伐他汀20mg组冠心病患者治疗前后血清TNF-α和LDL-C水平的变化。结果:阿托伐他汀10mg及20mg组患者治疗12周后血清TNF-α和LDL-C水平明显降低,并且阿托伐他汀20mg组比阿托伐他汀10mg组血清TNF-α下降更明显,差异有显著性(P<0.05),但两组间LDL-C水平降低无显著性差异(P>0.05)。结论:阿托伐他汀可以降低冠心病患者血清LDL-C和TNF-α水平,减轻冠心病的炎症反应,并且这种机制独立于降脂作用以外。     

120 例不稳定型心绞痛的地尔硫卓治疗临床观察

目的:探讨地尔硫卓治疗不稳定型心绞痛的临床疗效,以供医生参考使用。方法:将我院2010年1月~2011年5月收治的120例不稳定型心绞痛的患者随机分为两组,对照组患者给予硝酸甘油,实验组患者给予地尔硫卓,比较两组患者的治疗效果。结果:实验组患者的总有效率为93.33%(56/60),对照组患者总有效率为78.33%(47/60),实验组明显优于对照组患者,P<0.05。结论:地尔硫卓治疗不稳定型心绞痛具有较好的疗效,值得在临床应用。     

Effects of predation on host-pathogen dynamics in SIR models

The integration of infectious disease epidemiology with community ecology is an active area of research. Recent studies using SI models without acquired immunity have demonstrated that predation can suppress infectious disease levels. The authors recently showed that incorporating immunity (SIR models) can produce a “hump”-shaped relationship between disease prevalence and predation pressure; thus, low to moderate levels of predation can boost prevalence in hosts with acquired immunity. Here we examine the robustness of this pattern to realistic extensions of a basic SIR model, including density-dependent host regulation, predator saturation, interference, frequency-dependent transmission, predator numerical responses, and explicit resource dynamics. A non-monotonic relationship between disease prevalence and predation pressure holds across all these scenarios. With saturation, there can also be complex responses of mean host abundance to increasing predation, as well as bifurcations leading to unstable cycles (epidemics) and pathogen extinction at larger predator numbers. Firm predictions about the relationship between prevalence and predation thus require one to consider the complex interplay of acquired immunity, host regulation, and foraging behavior of the predator.     

Prenatal maternal programming determines testosterone response during social challenge

The present study investigated in domestic guinea pigs whether the effects of prenatal social stress are pathological consequences of adverse social conditions; or whether mothers adjust their offspring to the environment they experience during pregnancy. As a prenatal stressor social instability was used: we studied male guinea pig offspring whose mothers lived in a stable social environment (SE-sons) or in an unstable social environment (UE-sons) during pregnancy. Eight experimental groups were established, consisting of one SE-son, one UE-son and five females, respectively. All experimental groups remained in a stable group composition for the whole investigation time. We hypothesized that if mothers prenatally adapt their offspring, in a stable social environment SE-sons will be dominant, display agonistic and courtship behavior more frequently, have higher body weights, be less stressed and have higher testosterone concentrations than UE-sons. Our results show no significant differences between SE- and UE-sons concerning behavior, body weight or plasma-cortisol concentrations. Hence no evidence exists that an unstable social environment during pregnancy has pathological consequences for the male offsprings' phenotype. However, SE-sons had significantly higher plasma testosterone concentrations than UE-sons in phases when females were receptive. A higher reactivity of the hypothalamic-pituitary-gonadal axis might enable SE-males to adjust testosterone levels to the present social situation: they are significantly elevated in decisive phases of female receptivity, but remain on a lower level before and after reproductive challenge. Thus, mothers who experienced social stability during pregnancy provide their sons prenatally with a promising reproductive strategy in competitive situations later in life.     

参芍片联合酒石酸美托洛尔片对冠心病心绞痛患者氧化应激、血管内皮功能和心肌损伤标志物的影响

摘要 目的：探讨参芍片联合酒石酸美托洛尔片对冠心病心绞痛患者氧化应激、血管内皮功能和心肌损伤标志物的影响。方法：病例选取自我院2018年9月~2021年7月期间收治的110例冠心病心绞痛患者,按照入院的奇偶顺序将患者分为对照组（55例）和观察组（55例）,对照组患者接受酒石酸美托洛尔片治疗,观察组患者接受参芍片联合酒石酸美托洛尔片治疗,观察两组临床总有效率、心电图总有效率,对比两组临床症状、氧化应激[超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH-PX）]、血管内皮功能[内皮素（ET）、血管内皮生长因子（VEGF）、一氧化氮（NO）]和心肌损伤标志物[心肌肌钙蛋白（cTn）、肌酸激酶同工酶（CK-MB）、脑钠肽（BNP）]水平变化,记录两组用药的不良反应发生率。结果：与对照组相比,观察组的临床总有效率、心电图总有效率进一步升高（P<0.05）。观察组治疗12周后心绞痛发作次数较对照组少,心绞痛持续时间较对照组短,6 min步行试验距离长于对照组（P<0.05）。治疗12周后,观察组VEGF、ET水平低于对照组,NO水平高于对照组（P<0.05）。治疗12周后,观察组CK-MB、cTn、BNP水平低于对照组（P<0.05）。治疗12周后,观察组MDA水平低于对照组,SOD、GSH-PX水平高于对照组（P<0.05）。两组不良反应发生率组间对比无差异（P>0.05）。结论：参芍片联合酒石酸美托洛尔片治疗冠心病心绞痛患者,可促进症状改善,减轻机体氧化应激和血管内皮损伤,发挥较好的心肌保护作用。     

银杏酮酯滴丸联合美托洛尔对冠心病不稳定型心绞痛患者心肌酶指标、氧化应激和外周血IL-33/ST2信号通路的影响

摘要 目的：观察银杏酮酯滴丸联合美托洛尔对冠心病不稳定型心绞痛（UAP）患者心肌酶指标、氧化应激和外周血白介素-33（IL-33）/生长刺激表达基因2蛋白（ST2）信号通路的影响。方法：选取我院2020年1月~2021年12月期间收治的UAP患者70例,根据随机数字表法分为对照组（美托洛尔治疗,35例）和联合组（银杏酮酯滴丸联合美托洛尔治疗,35例）,对比两组疗效、心绞痛改善情况、心肌酶指标、氧化应激及外周血IL-33/ST2信号通路相关指标,记录不良反应发生情况。结果：联合组的临床总有效率（88.57%）高于对照组（68.57%）,差异有统计学意义（P<0.05）。治疗1个月后,联合组的心绞痛发作次数少于对照组,心绞痛发作持续时间短于对照组（P<0.05）。治疗1个月后,联合组血清肌酸激酶同工酶（CK-MB）、乳酸脱氢酶（LDH）、肌酸激酶（CK）、心肌肌钙蛋白（cTnI）低于对照组（P<0.05）。治疗1个月后,联合组血清丙二醛（MDA）水平低于对照组,血清超氧化物歧化酶（SOD）、总抗氧化能力（T-AOC）水平高于对照组（P<0.05）。治疗1个月后,联合组IL-33、ST2信使核糖核酸（mRNA）相对表达量低于对照组（P<0.05）。两组不良反应发生率组间对比无统计学差异（P>0.05）。结论：银杏酮酯滴丸联合美托洛尔治疗UAP患者,可促进症状改善,减轻心肌损伤和氧化应激,作用机制可能与调节IL-33/ST2信号通路有关。     

Periodic dynamics in a two-stage Allee effect model are driven by tension between stage equilibria

Single species difference population models can show complex dynamics such as periodicity and chaos under certain circumstances, but usually only when rates of intrinsic population growth or other life history parameter are unrealistically high. Single species models with Allee effects (positive density dependence at low density) have also been shown to exhibit complex dynamics when combined with over-compensatory density dependence or a narrow fertility window. Here we present a simple two-stage model with Allee effects which shows large amplitude periodic fluctuations for some initial conditions, without these requirements. Periodicity arises out of a tension between the critical equilibrium of each stage, i.e. when the initial population vector is such that the adult stage is above the critical value, while the juvenile stage is below the critical value. Within this area of parameter space, the range of initial conditions giving rise to periodic dynamics is driven mainly by adult mortality rates. Periodic dynamics become more important as adult mortality increases up to a certain point, after which periodic dynamics are replaced by extinction. This model has more realistic life history parameter values than most 'chaotic' models. Conditions for periodic dynamics might arise in some marine species which are exploited (high adult mortality) leading to recruitment limitation (low juvenile density) and might be an additional source of extinction risk.     

阿司匹林联合氯吡格雷治疗不稳定型心绞痛200例临床疗效分析

目的:探讨阿司匹林联合氯吡格雷治疗不稳定型心绞痛临床效果。方法:选取我院2012年7月-2013年6月不稳定型心绞痛患者200例,其中88例设置为对照组,在常规治疗基础上应用阿司匹林;观察组112例,在对照组基础上联合应用氯吡格雷治疗。结果:观察组效果评分为3.87±0.03,高于对照组3.24±0.05(P0.05);观察组心绞痛持续时间和心绞痛发作频率分别为3.18±0.03 min和1.39±0.02次/d,均低于对照组3.62±0.07 min和2.81±0.03次/d(P0.05);观察组血浆黏度、红细胞压积和红细胞聚积指数分别为0.78±0.02 P a o s,0.33±0.02 L/L和7.13±0.04(P0.05),均低于对照组(P0.05);两组结果比较差异均有统计学意义(P0.05);两组不良反应结果比较差异均无统计学意义(P0.05)。结论:阿司匹林联合氯吡格雷治疗不稳定型心绞痛疗效确切,可以进一步提高患者血流变学指标。     

Identification of an unstable 4-hydroxynoneal modification on the 20S proteasome subunit α7 by recombinant antibody technology.

Numerous cellular functions rely on an active proteasome allowing degradation of damaged or misfolded proteins. Therefore changes in the proteasomal activity have important physiological consequences. During oxidative stress the production of free radicals can result in the formation of 4-hydroxynonenal (HNE) following lipid peroxidiation. The HNE moiety is highly reactive and via a nucleophilic attack readily forms covalent links to cysteine, histidine and lysine side chains. However, as the chemical properties of these amino acids differ, so does the kinetics of the reactions. While covalent linkage through Michael addition is well established, reversible and unstable associations have only been indicated in a few cases. In the present study we have identified an unstable HNE adduct on the α7 subunit of the 20S proteasome using phage display of recombinant antibodies. This recombinant antibody fragment recognized HNE modified proteasomes in vitro and showed that this epitope was easily HNE modified, yet unstable, and influenced by experimental procedures. Hence unstable HNE-adducts could be overlooked as a regulatory mechanism of proteasomal activity and a participating factor in the decreased proteasomal activity associated with oxidative stress.     

超声检查颈动脉粥样硬化情况和肱动脉FMD 对稳定性心绞痛的价值

目的:探讨无创超声检查颈动脉粥样硬化情况和肱动脉FMD对SAP的价值。方法:将选取的65例SAP患者分为轻度狭窄组、中度狭窄组和重度狭窄组,超声检测所有病例的肱动脉FMD和颈总动脉IMT,并作Califf危险评分和As积分,分析观察指标间的关系。结果:Califf危险评分和As积分都随着冠脉狭窄程度的加深而增大,且二者呈正相关(P<0.05);颈总动脉IMT随着狭窄程度的加深而增大,肱动脉FMD则随着狭窄程度的加深而降低,二者呈负相关(P<0.05)。结论:无创监测颈总动脉IMT和肱动脉FMD,可指导SAP临床风险的控制。